D2000 DNA Ladder

● 產(chǎn)品編號(hào)及規(guī)格：

    RTM415-02       100T(2×250μl)      

● 產(chǎn)品簡介：

本產(chǎn)品是由6條帶狀雙鏈DNA條帶組成，適用于瓊脂糖凝膠電泳中DNA條帶的分析。產(chǎn)品中已含有1×loading buffer，可根據(jù)實(shí)驗(yàn)需要，直接取2-5μl電泳，使用方便，電泳圖像清晰。6條帶的大小分別為100，250，500，750，1000，2000bp。其中750bp條帶為100ng/5μl，顯示加亮帶，其余條帶濃度50ng/5μl。

● 使用方法：

1. 取2-5μl本產(chǎn)品加入到瓊脂糖凝膠的加樣孔中（每1mm×1mm加樣孔加1μl，如果加樣孔寬于

5mm，可以適當(dāng)增加上樣量）就行電泳。

2. 建議電泳條件：凝膠濃度為1.5-2.0％，凝膠長度5-7cm，電泳電壓4-10v/cm，電泳時(shí)間15-20

分鐘。

3. 通過EB染色后紫外燈下觀察條帶。

注：如果使用Goldview等染料就行染色，由于靈敏度比EB低，請(qǐng)酌情增加Marker的上樣量。

● 注意事項(xiàng)：

使用RTM415  D2000 DNA ladder產(chǎn)品發(fā)表部分文章列表：

1. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

2. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

3. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

4. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

5. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

6. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

7. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5