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首頁 > 核酸生物學 > 核酸電泳 > 雙鏈DNA Marker

1kb DNA ladder(1000-10000bp)試用裝

1kb DNA ladder(1000-10000bp)試用裝

產品編號:RTM417-S

產品規(guī)格:20次

相關規(guī)格:
數(shù)量
價格 ¥0

精準定量1kb DNA Ladder

● 產品編號及規(guī)格:

    RTM417-02       100T(2×250μl)

● 產品簡介:

    本產品是由8條帶狀雙鏈DNA條帶組成,適用于瓊脂糖凝膠電泳中DNA條帶的分析。

本產品為即用型產品,已含有1×loading buffer,可根據(jù)實驗需要,直接取2-5μl電泳,使用方便,電泳圖像清晰。本產品中8條帶分為1000(50 ng/5 μl),2000(50 ng/5 μl),3000(50 ng/5 μl),4000(100 ng/5 μl),5000(50 ng/5 μl),6000(50 ng/5 μl),8000(50 ng/5 μl),10000bp(50 ng/5 μl)。其中4000bp條帶加亮顯示。


● 儲存及運輸:

4貯存3個月, -20長期貯存;常溫運輸。

● 使用方法:

1. 取2-5μl本產品加入到瓊脂糖凝膠的加樣孔中(每1mm×1mm加樣孔加1μl,如果加樣孔寬于5mm,可以適當增加上樣量)就行電泳。

2. 建議電泳條件:凝膠濃度為0.8-1.0%,凝膠長度10 cm,電泳電壓~7v/cm,電泳時間60+分鐘。

3. 通過核酸染料染色后紫外燈或藍光儀下觀察條帶。


● 注意事項:

1. 瓊脂糖的質量對DNA的電泳有很大影響,電泳時請盡量使用質量優(yōu)等的瓊脂糖。由于PAGE電泳的特殊性,該產品不建議用于PAGE電泳。

2. 使用與電泳緩沖液一致的緩沖液融化瓊脂糖,例如使用1×TAE緩沖液電泳,需使用1×TAE緩沖液溶膠。

3. 多次電泳后緩沖液電導增強,相同電壓下電流增大,導致分辨率降低,并且明顯產熱,嚴重時會引起凝膠熔化或DNA變性。

4. 推薦使用RealSafe系列染料(貨號:GR002,GR003,GR004)染色。如果使用Goldview或EB等染料進行染色,由于此類染料帶更多的正電荷(染料移動方向與核酸泳動方向相反),隨著電泳時間延長,染料向大片段聚集,凝膠會出現(xiàn)陰陽膠現(xiàn)象(染料含量高的凝膠紫外下較亮,含量低的凝膠紫外下發(fā)暗),導致小分子量DNA顯色很弱或不可見。陰陽膠可以在電泳結束后浸泡染色,將膠浸沒在含1 μg /ml EB或Goldview的電泳緩沖液中20-25 min,小分子量DNA會顯色清楚。更長時間浸泡會因DNA分子擴散而使條帶彌散,小分子量DNA更易彌散。

5. 紫外照射下EB易分解,使DNA條帶熒光變淺。DNA受紫外光照射形成嘧啶二聚體,不利于待回收片段的下游工作,因此盡可能減少紫外照射時間。

6. 蔗糖會結合DNA,降低其電泳遷移率,建議DNA樣品上樣使用含甘油的Loading Buffer。

7. 大部分核酸工具酶會結合DNA,降低其電泳遷移率,大分子量DNA尤為明顯。

● 實驗示例:


● 附錄:

DNA在瓊脂糖凝膠中的有效分離范圍

瓊脂糖濃度%w/v

雙鏈DNA有效分離范圍(bp

優(yōu)選電泳緩沖液

示蹤染料相當于雙鏈DNA片段粗略大小(bp)

溴酚藍

二甲苯菁

TBE

TAE

TBE

TAE

0.5

2000-50000

1×TAE

750

1150

13000

16700

0.8

800-10000

1×TAE

320

530

4830

6500

1.0

400-8000

1×TAE

220

370

3030

4160

1.2

300-7000

1×TAE/0.5×TBE

160

275

2070

2890

1.5

200-3000

1×TAE/0.5×TBE

110

190

1300

1840

2.0

100-2000

0.5×TBE

65

120

710

1040

3.0

25-1000

0.5×TBE

30

60

300

460

 

 


使用DNA Marker產品發(fā)表部分文章列表:

1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415,D2000 DNA ladder

Journal: Phytotaxa. 2021,483 (2): 085–094.

Institution:University of Electronic Science and Technology of China

Paper linkhttps://doi.org/10.11646/phytotaxa.483.2.1

 

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403,DNA Marker III

Author: ZihanSong,ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution Zhejiang University

Paper linkhttps://doi.org/10.1016/j.ijbiomac.2021.11.194

 

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404,DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos one,January 2012 | Volume 7 | Issue 1 | e29875

InstitutionState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029875

 

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409,100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos one,September 2013 | Volume 8 | Issue 9 | e737

InstitutionSchool of Agriculture and Biology, Shanghai Jiao Tong University

Paper linkhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073729

 

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409,100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu,Z.-L. Zhang

Journal: Plant Biology,14 (2012) 828–838

InstitutionKey Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper linkhttps://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00559.x

 

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415,D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One,Published: December 21, 2015

InstitutionKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

 

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415,D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER,ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa,2015,227 (3): 229–242

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper linkhttps://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

 

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415,D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper linkhttps://link.springer.com/article/10.1007/s13225-012-0190-9

 

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421,λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

InstitutionKey Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link

https://www.sciencedirect.com/science/article/abs/pii/S0003269718310637?via%3Dihub

 

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415,D2000 DNA ladder

Author: Huan Jiang,Wenxing Long, Hui Zhang,Chengneng Mi,Tao Zhou,Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

InstitutionInstitute of Tropical Agriculture and Forestry, Hainan University

Paper link https://doi.org/10.1002/ece3.5127

 

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406,DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

InstitutionState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link https://www.frontiersin.org/articles/10.3389/fmicb.2019.01076/full

 

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415,D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7,2021,3071-3077

InstitutionBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper linkhttps://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

 

 

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415,D2000 DNA ladder

Author: Kevin D Hyde,Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

InstitutionSchool of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper linkhttps://link.springer.com/article/10.1007/s13225-021-00495-5


 
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